Location of a membrane-bound carbonic anhydrase isoenzyme (CA IV) in the human male reproductive tract.
نویسندگان
چکیده
منابع مشابه
Secretory carbonic anhydrase isoenzyme (CA VI) in human serum.
Carbonic anhydrase VI (CA VI) is a secretory isoenzyme that, by analogy to alpha-amylase, is produced in the salivary glands and delivered into saliva. To determine whether CA VI is transferred into the circulation and is detectable in human serum, we collected blood samples from four healthy subjects at 3-h intervals throughout a 24-h period and measured concentrations of CA VI by a specific t...
متن کاملInterstitial carbonic anhydrase (CA) activity in brain is attributable to membrane-bound CA type IV.
We tested the hypothesis that extracellular membrane-bound carbonic anhydrase (CA) type IV is responsible for the regulation of interstitial pH (pH(o)) transients in brain. Rat hippocampal slices were incubated in phosphatidylinositol-specific phospholipase C (PI-PLC), which cleaves the link of CA IV to the external face of plasma membranes. Then evoked alkaline pH(o) shifts were studied in a r...
متن کاملTissue and species distribution of the secreted carbonic anhydrase isoenzyme.
The secreted carbonic anhydrases, CA VI, are high molecular mass, oligomeric enzymes originally found in the sheep parotid gland and saliva. The enzymes have been purified from the saliva or parotid glands of several different species. All the CA VI enzymes studied have an apparent subunit Mr of about 45,000 as previously reported for the sheep enzyme. By Western analysis, CA VI from human, cow...
متن کاملP-17: Expression of Cell Surface Toll-Like Receptors in the Human Male Reproductive Tract
Background: Male infertility refers to the inability of a male to achieve a pregnancy in a fertile female. The root of many causes of infertility is miscommunication between immune and reproductive system. Male reproductive system is very sensitive and vulnerable, infections can hinder maturation and movement of spermatozoa lead to impaired fertility.All species need an immediate reply to the m...
متن کاملCatalysis and inhibition of human carbonic anhydrase IV.
Carbonic anhydrase IV (CA IV) is a membrane-bound form of carbonic anhydrase. We have characterized the catalytic activity and inhibition of recombinant human CA IV. CA IV is a high-activity isozyme in CO2 hydration with a pH-independent kcat value (1.1 x 10(6) s(-1)) comparable to that of CA II (8 x 10(5) s(-1)). Furthermore, CA IV is more active in HCO3- dehydration than is CA II as illustrat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1993
ISSN: 0022-1554,1551-5044
DOI: 10.1177/41.5.8468457